![]() ![]() ![]() The first is: “All tissues from immunocompromised (including corticosteroid-treated) patients and those with suspected fungal infection should be stained with fungal stains such as periodic acid-Schiff, silver, or fluorescent stains, in parallel with regular stains. The BSMM Standards of Care (2003) set out 2 standards for histopathology. Yeasts: “Histopathologic, cytopathologic, or direct microscopic examination of a specimen obtained by needle aspiration or biopsy from a normally sterile site (other than mucous membranes) showing yeast cells-for example, Cryptococcus species indicated by encapsulated budding yeasts or Candida species showing pseudohyphae or true hyphae.” Moulds: “Histopathologic, cytopathologic, or direct microscopic examination of a specimen obtained by needle aspiration or biopsy in which hyphae or melanized yeast-like forms are seen accompanied by evidence of associated tissue damage.” So the 2008 EORTC/MSG definitions of invasive fungal infection carry these statements for proven infections Demonstration of fungi in tissue is confirmatory of fungal infection. ![]()
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